Wnt Signaling: Methods and Protocols by Quinn Barrett (English) Hardcover Book-image

Wnt Signaling: Methods and Protocols by Quinn Barrett (English) Hardcover Book

$ 136.88

Publication Name: NA ISBN-13: 9781493963911 gtin13: 9781493963911 ISBN: 9781493963911 Type: NA

Description

The Nile on eBay FREE SHIPPING UK WIDE Wnt Signaling by Quinn Barrett, Lawrence Lum Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. FORMAT Hardcover LANGUAGE English CONDITION Brand New Publisher Description Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. The methodologies include using Wnt modulating chemicals in engineering tissues from induced pluripotent and embryonic stem cells, monitoring Wnt transcriptional responses in diverse tissues such as bone and skin, and using specific biochemical markers of Wnt signaling to either screen molecular libraries or evaluate novel reagents. These protocols also leverage unique experimental strengths from five different model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Wnt Signaling: Methods and Protocols aims to support researchers in understanding Wnt signal transduction in any effort to improve outcomes in regenerative medicine and cancer management. Back Cover Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. The methodologies include using Wnt modulating chemicals in engineering tissues from induced pluripotent and embryonic stem cells, monitoring Wnt transcriptional responses in diverse tissues such as bone and skin, and using specific biochemical markers of Wnt signaling to either screen molecular libraries or evaluate novel reagents. These protocols also leverage unique experimental strengths from five different model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Wnt Signaling: Methods and Protocols aims to support researchers in understanding Wnt signal transduction in any effort to improve outcomes in regenerative medicine and cancer management. Table of Contents Visualizing Wnt Palmiteoylation in Single Cells.- Monitoring Wnt Protein Acylation Using an In Vitro Cyclo-Addition Reaction.- Biochemical Methods to Analyze Wnt Protein Secretion.- Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and Notum.- Probing Wnt Receptor Turnover: A Critical Regulatory Point of Wnt Pathway.- A Simple Method to Assess Abundance of the b-Catenin Signaling Pool in Cells.- Wnt-Dependent Control of Cell Polarity in Cultured Cells.- The Use of Chick Embryos to Study Wnt Signaling/Gradients.- Monitoring Wnt Signaling in Zebrafish Using Fluorescent Biosensors.- Biochemical Analysis of Tankyrase Activity in Zebrafish In Vitro and In Vivo.- Reconstitution of the Cytoplasmic Regulation of the Wnt Signaling Pathway Using Xenopus Egg Extracts.- Delivery of the Porcupine Inhibitor WNT974 in Mice.- Use of Primary Calvarial Osteoblasts to Evaluate the Function of Wnt Signaling in Osteogenesis.- Monitoring Wnt/b-Catenin Signaling in Skin.- The Generation of Organoids for Studying Wnt Signaling.- Methods to Manipulate and Monitor Wnt Signaling in Human Pluripotent Stem Cells.- Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions. Feature Includes cutting-edge methods and protocols for the study of Wnt signaling Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Details ISBN 1493963910 Short Title WNT SIGNALING 2016/E Language English ISBN-10 1493963910 ISBN-13 9781493963911 Media Book Format Hardcover DEWEY 571.6 Series Number 1481 Year 2016 Publication Date 2016-09-03 Imprint Humana Press Inc. Subtitle Methods and Protocols Place of Publication Totowa, NJ Country of Publication United States Edited by Lawrence Lum Edition 1st DOI 10.1007/978-1-4939-6393-5 AU Release Date 2016-09-03 NZ Release Date 2016-09-03 US Release Date 2016-09-03 UK Release Date 2016-09-03 Author Lawrence Lum Birth 1979 Affiliation Harvard University Position Graduate Student Qualifications M.D. Pages 198 Publisher Humana Press Inc. Edition Description 1st ed. 2016 Series Methods in Molecular Biology Alternative 9781493981854 Audience Professional & Vocational Illustrations 32 Illustrations, color; 13 Illustrations, black and white; XI, 198 p. 45 illus., 32 illus. in color. Country of Origin NL Product Class Description Life Sciences: General We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! 30 DAY RETURN POLICY No questions asked, 30 day returns! FREE DELIVERY No matter where you are in the UK, delivery is free. SECURE PAYMENT Peace of mind by paying through PayPal and eBay Buyer Protection TheNile_Item_ID:137618612;

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