Sphingosine-1-Phosphate: Methods and Protocols by Kursad Turksen (English) Paper

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The Nile on eBay FREE SHIPPING UK WIDE Sphingosine-1-Phosphate by Kursad Turksen, Alice Pébay This Methods in Molecular Biology(TM) volume describes in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology. Includes troubleshooting tips and more. FORMAT Paperback LANGUAGE English CONDITION Brand New Publisher Description Sphingosine-1-phosphate is a bioactive lysophospholipid which has become, in recent years, the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells. In Sphingosine-1-Phosphate: Methods and Protocols, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology. As a volume in the Methods in Molecular Biology™ series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and timely, Sphingosine-1-Phosphate: Methods and Protocols is a key resource for scientists working in this expanding and dynamic field. Back Cover Sphingosine-1-phosphate is a bioactive lysophospholipid which has become, in recent years, the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells. In Sphingosine-1-Phosphate: Methods and Protocols , worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology. As a volume in the Methods in Molecular Biology(tm) series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and timely, Sphingosine-1-Phosphate: Methods and Protocols is a key resource for scientists working in this expanding and dynamic field. Table of Contents Biochemical Methods for Quantifying Sphingolipids: Ceramide, Sphingosine, Sphingosine Kinase-1 Activity, and Sphingosine-1-Phosphate.- Isoform-Selective Assays for Sphingosine Kinase Activity.- Quantification of Sphingosine-1-Phosphate and Related Sphingolipids by Liquid Chromatography Coupled to Tandem Mass Spectrometry.- A Clean-up Method for Mass Spectrometric Analysis of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule.- Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples.- Assessment of Sphingosine-1-Phosphate Activity in Biological Samples by Receptor Internalization and Adherens Junction Formation.- High Throughput Assays to Measure Intracellular Ca2+ Mobilization in Cells that Express Recombinant S1P Receptor Subtypes.- Quantifying Sphingosine-1-Phosphate-Dependent Activation of the RhoGTPases.- Optimized Protocols to Analyze Sphingosine-1-Phosphate Signal Transduction Pathways During Acrosomal Exocytosis in Human Sperm.- Use of Intravital Microscopy and In Vitro Chemotaxis Assays to Study the Roles of Sphingosine-1-Phosphate in Bone Homeostasis.- Assessment of Sphingosine-1-Phosphate Receptor Expression and Associated Intracellular Signaling Cascades in Primary Cells of the Human Central Nervous System.- Sphingosine-1-Phosphate Signaling in Skeletal Muscle Cells.- Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor.- Ceramide and Sphingosine-1-Phosphate Signaling in Embryonic Stem Cell Differentiation.- Sphingosine-1-Phosphate: An Alternative to Serum for Culture and Multipotency Maintenance of Adult Human Mesenchymal Stem Cells.- Sphingosine-1-Phosphate Signaling in Neural Progenitors.- Utilizing Sphingosine-1-Phosphate to Stimulate Sprouting Angiogenesis. Feature Features the important effects of S1P in cell development and physiopathology Includes detailed, tried-and-true protocols, ready for the lab Contains expert tips and key troubleshooting advice to ensure successful results Details ISBN 1493959638 Pages 221 Publisher Humana Press Inc. Series Methods in Molecular Biology Year 2016 ISBN-10 1493959638 ISBN-13 9781493959631 Format Paperback Publication Date 2016-08-23 Imprint Humana Press Inc. Subtitle Methods and Protocols Place of Publication Totowa, NJ Country of Publication United States DEWEY 572.548 Edited by Alice Pébay Affiliation Loeb Health Research Instituteottawa Hospital Short Title Sphingosine-1-Phosphate Language English Series Number 874 UK Release Date 2016-08-23 AU Release Date 2016-08-23 NZ Release Date 2016-08-23 US Release Date 2016-08-23 Author Alice Pébay Edition Description Softcover reprint of the original 1st ed. 2012 Alternative 9781617797996 Audience Professional & Vocational Illustrations 10 Illustrations, color; 29 Illustrations, black and white; XI, 221 p. 39 illus., 10 illus. in color. Country of Origin NL Product Class Description Science & Mathematics: Textbooks & Study Guides We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! 30 DAY RETURN POLICY No questions asked, 30 day returns! 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