Mouse Cell Culture: Methods and Protocols by Andrew Ward (English) Paperback Boo

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The Nile on eBay FREE SHIPPING UK WIDE Mouse Cell Culture by Andrew Ward, David Tosh In this book, expert researchers provide a number of methods for the culture of a range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Notes provide practical, straight-from-the-lab knowledge and experiences. FORMAT Paperback LANGUAGE English CONDITION Brand New Publisher Description Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders. Back Cover Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology(tm) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders. Table of Contents The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies.- Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures.- Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells.- The Embryonic Kidney: Isolation, Organ Culture, Immunostaining and RNA Interference.- Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium, or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation.- Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine.- Isolation and Culture of Embryonic Pancreas and Liver.- Isolation and Culture of Mouse Satellite Cells.- Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures.- Short- and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes.- Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells.- Isolation and Culture of Mouse Pancreatic Islets for Ex Vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes.- Isolation and Culture of Adult Mouse Hepatocytes.- Isolation and Culture of Mouse Intestinal Cells.- Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse.- The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent Cytochemistry.- Cell Culture of Primary Cerebellar Granule Cells.- Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain. Review From the reviews:"This book provides a diverse collection of protocols for mouse cell culture in a text comprising 18 chapters. … This book is recommended for researchers and students who are interested in studying the culture of cells from animal models." (Elvira M. Guerra Shinohara, Brazilian Journal of Pharmaceutical Sciences, October-December, 2010) Long Description Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology(tm) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders. Review Quote From the reviews: "This book provides a diverse collection of protocols for mouse cell culture in a text comprising 18 chapters. ... This book is recommended for researchers and students who are interested in studying the culture of cells from animal models." (Elvira M. Guerra Shinohara, Brazilian Journal of Pharmaceutical Sciences, October-December, 2010) Feature Features methods involving a wide variety of tissue types in one convenient volume Written by leading experts in an easy-to-use step-by-step format with notes providing practical, straight-from-the-lab knowledge and experiences Covers protocols with fetal tissues and stem cells as well as cultures of isolated cell types for the study of developmental processes and homogeneous cell populations, respectively Details ISBN 1493960865 Pages 257 Publisher Humana Press Inc. Series Methods in Molecular Biology Year 2016 ISBN-10 1493960865 ISBN-13 9781493960866 Format Paperback Publication Date 2016-08-23 Imprint Humana Press Inc. Subtitle Methods and Protocols Place of Publication Totowa, NJ Country of Publication United States Edited by David Tosh DEWEY 571.63819353 Short Title Mouse Cell Culture Language English Series Number 633 UK Release Date 2016-08-23 AU Release Date 2016-08-23 NZ Release Date 2016-08-23 US Release Date 2016-08-23 Author David Tosh Edition Description Softcover reprint of the original 1st ed. 2010 Alternative 9781588297723 Audience Professional & Vocational Illustrations XI, 257 p. Country of Origin NL Product Class Description Science & Mathematics: Textbooks & Study Guides We've got this At The Nile, if you're looking for it, we've got it. 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