siRNA Design: Methods and Protocols by Debra J. Taxman (English) Paperback Book

Description

The Nile on eBay FREE SHIPPING UK WIDE siRNA Design by Debra J. Taxman The discovery of RNA interference (RNAi) as a methodology for gene silencing has revolutionized biological research, providing an invaluable avenue for therapeutics, and small interfering RNA (siRNA) is the most common strategy utilized for enacting RNAi. FORMAT Paperback LANGUAGE English CONDITION Brand New Publisher Description The discovery of RNA interference (RNAi) as a methodology for gene silencing has revolutionized biological research, providing an invaluable avenue for therapeutics, and small interfering RNA (siRNA) is the most common strategy utilized for enacting RNAi. siRNA Design: Methods and Protocols offers expertly crafted guidelines and protocols for the selection of siRNA targeting sequences, for the strategic incorporation of chemical modifications, and for advantageous structural modifications to the classic siRNA design. Protocols are provided for using endogenous cellular machinery to produce siRNA from optimized precursor short hairpin RNA (shRNA) and artificial microRNA (amiRNA) molecules. Strategies are also described for specific applications such as immunostimulatory siRNA that may provide therapeutic benefit against viral infections in mammals, the simultaneous targeting of multiple siRNAs, and siRNA-mediated crop virus resistance. The design of RNAi for gene silencing in embryonic, invertebrate, and plant systems requires a variety of unique approaches, several of which are described towards the end of this volume. Written for the highly successful Methods in Molecular Biology™ series, this work contains the kind of detailed description and implementation advice that guarantees successful results. Authoritative and easy to use, siRNA Design: Methods and Protocols will provide researchers, educators, clinicians, and biotech specialists with a broad understanding of the issues in RNAi and how they can be overcome strategically through design. Back Cover The discovery of RNA interference (RNAi) as a methodology for gene silencing has revolutionized biological research, providing an invaluable avenue for therapeutics, and small interfering RNA (siRNA) is the most common strategy utilized for enacting RNAi. s iRNA Design: Methods and Protocols offers expertly crafted guidelines and protocols for the selection of siRNA targeting sequences, for the strategic incorporation of chemical modifications, and for advantageous structural modifications to the classic siRNA design. Protocols are provided for using endogenous cellular machinery to produce siRNA from optimized precursor short hairpin RNA (shRNA) and artificial microRNA (amiRNA) molecules. Strategies are also described for specific applications such as immunostimulatory siRNA that may provide therapeutic benefit against viral infections in mammals, the simultaneous targeting of multiple siRNAs, and siRNA-mediated crop virus resistance. The design of RNAi for gene silencing in embryonic, invertebrate, and plant systems requires a variety of unique approaches, several of which are described towards the end of this volume. Written for the highly successful Methods in Molecular Biology(tm) series, this work contains the kind of detailed description and implementation advice that guarantees successful results. Authoritative and easy to use, s iRNA Design: Methods and Protocols will provide researchers, educators, clinicians, and biotech specialists with a broad understanding of the issues in RNAi and how they can be overcome strategically through design. Table of Contents What Parameters to Consider and Which Software Tools to Use for Target Selection and Molecular Design of Small Interfering RNAs.- Methods for Selecting Effective siRNA Target Sequences Using a Variety of Statistical and Analytical Techniques.- Designing Functional siRNA with Reduced Off-Target Effects.- Design and Screening of siRNAs Against Highly Structured RNA Targets.- Engineering Small Interfering RNAs by Strategic Chemical Modification.- The Design, Selection and Evaluation of Highly Specific and Functional siRNA Incorporating Unlocked Nucleobase Analogs.- The Design, Preparation, and Evaluation of Asymmetric Small Interfering RNA for Specific Gene Silencing in Mammalian Cells.- Design of Nuclease-Resistant Fork-Like Small Interfering RNA (fsiRNA).- Designing Dual-Targeting siRNA Duplexes Having Two Active Strands that Combine siRNA and MicroRNA-Like Targeting.- Strategies for Designing and Validating Immunostimulatory siRNAs.- Designing Efficient and Specific Endoribonuclease Prepared siRNAs (esiRNAs).- Short Hairpin RNA-Mediated Gene Silencing.- Design of Lentivirally Expressed siRNAs.- Bi-Functional Short Hairpin RNA (bi-shRNA): Design and Pathway to Clinical Application.- Design and Chemical Modification of Synthetic Short shRNAs as Potent RNAi Triggers.- Production and Application of Long dsRNA in Mammalian Cells.- Design of RNAi Reagents for Invertebrate Model Organisms and Human Disease Vectors.- Construction of shRNA Expression Plasmids for Silkworm Cell Lines Using Single-Stranded DNA and Bst DNA Polymerase.- Designing Effective amiRNA and Multimeric amiRNA Against Plant Viruses.- Downregulation of Plant Genes with MiRNA Induced Gene Silencing. Review From the reviews:"This book edited by D.J. Taxman is a valuable collection of 20 chapters covering aspects from values for parameters used in tools for designing siRNAs to screening methods for evaluating the efficiency of the designed siRNA. … Through style and scientific information the book is aimed at researchers in the RNAi field, but its accessible delivery of contents makes it a good starting point for younger researchers in this topic." (Irina Ioana Mohorianu, zbMATH, Vol. 1276, 2014) Review Quote From the reviews: This book edited by D.J. Taxman is a valuable collection of 20 chapters covering aspects from values for parameters used in tools for designing siRNAs to screening methods for evaluating the efficiency of the designed siRNA. Through style and scientific information the book is aimed at researchers in the RNAi field, but its accessible delivery of contents makes it a good starting point for younger researchers in this topic. (Irina Ioana Mohorianu, zbMATH, Vol. 1276, 2014)" Feature Assists researchers, educators, clinicians, and biotech scientists in harnessing the power of RNAi Features easy to follow protocols and techniques for siRNA design Includes expert tips and implementation advice to ensure successful results Details ISBN 1493959786 Short Title SIRNA DESIGN Language English ISBN-10 1493959786 ISBN-13 9781493959785 Media Book Format Paperback DEWEY 572.88 Series Number 942 Year 2016 Subtitle Methods and Protocols Country of Publication United States Imprint Humana Press Inc. Place of Publication Totowa, NJ Edited by Debra J. Taxman Publication Date 2016-08-23 UK Release Date 2016-08-23 AU Release Date 2016-08-23 NZ Release Date 2016-08-23 US Release Date 2016-08-23 Author Debra J. Taxman Pages 392 Publisher Humana Press Inc. Edition Description Softcover reprint of the original 1st ed. 2013 Series Methods in Molecular Biology Alternative 9781627031189 Audience Professional & Vocational Illustrations XIII, 392 p. Country of Origin NL Product Class Description Engineering & Technology: Textbooks & Study Guides We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! 30 DAY RETURN POLICY No questions asked, 30 day returns! FREE DELIVERY No matter where you are in the UK, delivery is free. SECURE PAYMENT Peace of mind by paying through PayPal and eBay Buyer Protection TheNile_Item_ID:137767796;