Live Cell Imaging: Methods and Protocols by Sung-Bae Kim (English) Hardcover Boo

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The Nile on eBay FREE SHIPPING UK WIDE Live Cell Imaging by Sung-Bae Kim The chapters are categorized into seven major groups according to the reporter materials, such as imaging with passive optical readouts, activatable bioluminescent probes, functional substrates and luciferases, organic fluorescent probes, BRET probes, FRET probes, as well as with advanced instrumentation. FORMAT Hardcover LANGUAGE English CONDITION Brand New Publisher Description This detailed book highlights recent advances in molecular imaging techniques and protocols, designed to be immediately applicable in global bio-laboratories. The chapters are categorized into seven major groups according to the reporter materials, such as imaging with passive optical readouts, activatable bioluminescent probes, functional substrates and luciferases, organic fluorescent probes, BRET probes, FRET probes, as well as with advanced instrumentation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Live Cell Imaging: Methods and Protocols aims to direct and inspire researchers into creating smarter, next-generation imaging techniques that are truly quantitative, highly sensitive, and readily comprehended, in the effort to engender deeper understanding of biological systems and break new ground in the research fields of life science. Back Cover This detailed book highlights recent advances in molecular imaging techniques and protocols, designed to be immediately applicable in global bio-laboratories. The chapters are categorized into seven major groups according to the reporter materials, such as imaging with passive optical readouts, activatable bioluminescent probes, functional substrates and luciferases, organic fluorescent probes, BRET probes, FRET probes, as well as with advanced instrumentation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Live Cell Imaging: Methods and Protocols aims to direct and inspire researchers into creating smarter, next-generation imaging techniques that are truly quantitative, highly sensitive, and readily comprehended, in the effort to engender deeper understanding of biological systems and break new ground in the research fields of life science. Table of Contents Fluorescent Labeling of the Nuclear Envelope without Relying on Inner Nuclear Membrane Proteins.- Quantitative Analysis of Membrane Receptor Trafficking Manipulated by Optogenetic Tools.- Simultaneous Detection of Four Cell Cycle Phases with Live Fluorescence Imaging.- A Murine Bone Metastasis Model Using Caudal Artery Injection and Bioluminescence Imaging.- A New Lineage of Artificial Luciferases for Mammalian Cell Imaging.- Use of Bacterial Luciferase as a Reporter Gene in Eukaryotic Systems.- Quantitative Determination and Imaging of Gαq Signaling in Live Cells via Split-Luciferase Complementation.- A Split-Luciferase-Based Cell Fusion Assay for Evaluating the Myogenesis-Promoting Effects of Bioactive Molecules.- Development of a Single Fluorescent Protein-Based Green Glucose Indicator by Semi-Rational Molecular Design and Molecular Evolution.- Near Infrared Bioluminescence Imaging of Animal Cells with Through-Bond Energy Transfer Cassette.- Azide- and Dye-Conjugated Coelenterazine Analogues for Imaging Mammalian Cells.- Luciferase-Specific Coelenterazine Analogues for Optical Crosstalk-Free Bioassays.- Live Imaging of Virus-Infected Cells by Using a Sialidase Fluorogenic Probe.- Live-Cell Imaging of Sirtuin Activity Using a One-Step Fluorescence Probe.- Live-Cell Fluorescence Imaging of Microtubules by Using a Tau-Derived Peptide.- Discovery of the Environment-Sensitive Near-Infrared (NIR) Fluorogenic Ligand for α1-Adrenergic Receptors Imaging In Vivo.- Rapid and Sensitive Detection of Cancer Cells with Activatable Fluorescent Probes for Enzyme Activity.- Detection of Intracellular Reactive Oxidative Species Using the Fluorescent Probe Hydroxyphenyl Fluorescein.- Development of Near-Infrared Fluorescent Mg2+ Probe and Application to Multi-Color Imaging of Intracellular Signals.- Long-Term Mg2+ Imaging in Live Cells with a Targetable Fluorescent Probe.- Highly Bright NIR-BRET System for Imaging Molecular Events in Live Cells.- Ligand-Activatable BRET9 Probes for Imaging Molecular Events in Living Mammalian Cells.- Manipulation of Actin Cytoskeleton by Intracellular-Targeted ROS Generation.- Bioluminescence Imaging of Neuronal Network Dynamics Using Aequorin-Based Calcium Sensors.- Method for Detecting Emission Spectral Change of Bioluminescent Ratiometric Indicators via a Smartphone.- Bioluminescence Resonance Energy Transfer (BRET) Imaging in Living Cells: Image Acquisition and Quantification.- GPCR Signaling Regulation in Dictyostelium Chemotaxis.- Live Cell Imaging Technique to Visualize DAMPs Release during Regulated Cell Death.- Time-Lapse Imaging of Necroptosis and DAMP Release at Single Cell Resolution.- High-Throughput Whole Plate Imaging of Cells for Multiple Biological Applications.- Pinhole Closure Improves Spatial Resolution in Confocal Scanning Microscopy.- Total Workflows of the Single-Molecule Imaging Analysis in Living Cells: Tutorial Guidance to the Measurement of the Drug Effects on a GPCR. Feature Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts Details ISBN 1071612573 Short Title Live Cell Imaging Series Methods in Molecular Biology Language English Year 2021 ISBN-10 1071612573 ISBN-13 9781071612576 Format Hardcover Subtitle Methods and Protocols Series Number 2274 DOI 10.1007/978-1-0716-1258-3 Publisher Springer-Verlag New York Inc. Place of Publication New York, NY Country of Publication United States Pages 447 Publication Date 2021-05-29 AU Release Date 2021-05-29 NZ Release Date 2021-05-29 US Release Date 2021-05-29 UK Release Date 2021-05-29 Author Sung-Bae Kim Edited by Sung-Bae Kim DEWEY 571.6028 Audience Professional & Vocational Edition Description 2021 ed. Edition 2021st Country of Origin NL Product Class Description Life Sciences: General Imprint Humana Alternative 9781071612583 Illustrations 109 Illustrations, color; 29 Illustrations, black and white We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! 30 DAY RETURN POLICY No questions asked, 30 day returns! FREE DELIVERY No matter where you are in the UK, delivery is free. SECURE PAYMENT Peace of mind by paying through PayPal and eBay Buyer Protection TheNile_Item_ID:144039293;