Telomeres and Telomerase: Methods and Protocols by Zhou Songyang (English) Paper

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The Nile on eBay FREE SHIPPING UK WIDE Telomeres and Telomerase by Zhou Songyang In its updated 2nd Edition, this book builds on the telomerase assays featured in the popular first edition. Using the Methods in Molecular Biology(TM) format, it includes materials and reagents lists, step-by-step protocols, and expert tips on troubleshooting. FORMAT Paperback LANGUAGE English CONDITION Brand New Publisher Description New and rapid advances in technology have equipped us with a variety of tools and platforms to ask fundamental questions of telomere regulation and have allowed investigators to carry out experiments using diverse model systems. For example, proteomic, genomic, and molecular approaches have afforded us unprecedented insight into the complex protein interaction networks at work on the telomere chromatin and the detailed information regarding telomere dynamics in response to stress or stimuli. Telomeres and Telomerase: Methods and Protocols, Second Edition builds upon the telomerase assays featured in the popular first edition to encompass many different assays that allow investigators to query the function of telomere proteins and the responses of the telomere DNA, including detailed examinations of biochemical, molecular, and proteomic approaches. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Telomeres and Telomerase: Methods and Protocols, Second Edition serves as an ideal, up-to-date guide for investigators further pursing this vital field of study. Back Cover New and rapid advances in technology have equipped us with a variety of tools and platforms to ask fundamental questions of telomere regulation and have allowed investigators to carry out experiments using diverse model systems. For example, proteomic, genomic, and molecular approaches have afforded us unprecedented insight into the complex protein interaction networks at work on the telomere chromatin and the detailed information regarding telomere dynamics in response to stress or stimuli. Telomeres and Telomerase: Methods and Protocols, Second Edition builds upon the telomerase assays featured in the popular first edition to encompass many different assays that allow investigators to query the function of telomere proteins and the responses of the telomere DNA, including detailed examinations of biochemical, molecular, and proteomic approaches. Written in the highly successful Methods in Molecular Biology(tm) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Telomeres and Telomerase: Methods and Protocols, Second Edition serves as an ideal, up-to-date guide for investigators further pursing this vital field of study. Table of Contents Introduction to Telomeres and Telomerase.- Analysis of Average Telomere Length in Cultured Human Cells.- Telomere Length Analysis by Quantitative Fluorescent In situ Hybridization (Q-FISH).- Telomere Strand-Specific Length Analysis by Fluorescent In situ Hybridization (Q-CO-FISH).- Telomere G-Overhang Length Measurement Method 1: The DSN Method.- Telomere G-Overhang Length Measurement Method 2: G-Tail Telomere HPA.- Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-rich Fill-In.- G-Quadruplex Structures and G-Quadruplex-Interactive Compounds.- Analysis of Yeast Telomerase by Primer Extension Assays.- Telomeric Repeat Amplification Protocol (TRAP): Measuring the Activity of the Telomerase.- CO-FISH, COD-FISH, ReD-FISH, SKY-FISH.- Visualization of Human Telomerase Localization by Fluorescence Microscopy Techniques.- Cytogenetic Analysis of Telomere Dysfunction.- Probing the Telomere Damage Response.- Analysis of Telomere Proteins by Chromatin Immunoprecipitation (ChIP).- Studying of Telomeric Protein-Protein Interactions by Bi-Molecular Fluorescence Complementation (BiFC) and Peptide Array-Based Assays.- Human Telomere POT1-TPP1 Complex and Its Role in Telomerase Activity Regulation. Review From the reviews of the second edition:"The book provides different assays to study the function of telomere proteins and the responses of the telomere DNA. It also provides protocols for biochemical, molecular, and proteomic approaches in the understanding of telomere dynamics. It appears to be intended for investigators interested in the field of telomere and telomerase, but it also will be of immense help to other clinicians interested in cell growth, proliferation, aging, and cancer." (Omer Iqbal, Doody's Book Reviews, February, 2012) Review Quote From the reviews of the second edition: "The book provides different assays to study the function of telomere proteins and the responses of the telomere DNA. It also provides protocols for biochemical, molecular, and proteomic approaches in the understanding of telomere dynamics. It appears to be intended for investigators interested in the field of telomere and telomerase, but it also will be of immense help to other clinicians interested in cell growth, proliferation, aging, and cancer." (Omer Iqbal, Doody's Book Reviews, February, 2012) Feature Features cutting-edge protocols from this expanding and celebrated field of study Details biochemical, molecular, and proteomic approaches Contains expert tips and key implementation advice Details ISBN 1493956272 Pages 191 Publisher Humana Press Inc. Series Methods in Molecular Biology Year 2016 ISBN-10 1493956272 ISBN-13 9781493956272 Format Paperback Publication Date 2016-08-23 Imprint Humana Press Inc. Subtitle Methods and Protocols Place of Publication Totowa, NJ Country of Publication United States Edited by Zhou Songyang DEWEY 574.8 Short Title Telomeres and Telomerase Language English Edition 2nd Series Number 735 UK Release Date 2016-08-23 AU Release Date 2016-08-23 NZ Release Date 2016-08-23 US Release Date 2016-08-23 Illustrations X, 191 p. Author Zhou Songyang Edition Description Softcover reprint of the original 2nd ed. 2011 Alternative 9781617790911 Audience Professional & Vocational Country of Origin NL Product Class Description Science & Mathematics: Textbooks & Study Guides We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! 30 DAY RETURN POLICY No questions asked, 30 day returns! FREE DELIVERY No matter where you are in the UK, delivery is free. 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