Hypoxia: Methods and Protocols by L. Eric Huang (English) Hardcover Book-image

Hypoxia: Methods and Protocols by L. Eric Huang (English) Hardcover Book

$ 107.77

gtin13: 9781493976645 ISBN-13: 9781493976645 Publication Name: NA Type: NA ISBN: 9781493976645

Description

The Nile on eBay FREE SHIPPING UK WIDE Hypoxia by L. Eric Huang This volume details widely used and newer lab protocols for studying hypoxic responses in physiology and diseases. FORMAT Hardcover LANGUAGE English CONDITION Brand New Publisher Description This volume details widely used and newer lab protocols for studying hypoxic responses in physiology and diseases. Chapters guide the reader through the application of hypoxic conditions, to the techniques of molecular biology, biochemical, cell biology, genomic,bioinformatic, metabolic, and animal studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Hypoxia: Methods and Protocols aims to provide a valuable set of tools that can be used to study hypoxia and beyond. Back Cover This volume details widely used and newer lab protocols for studying hypoxic responses in physiology and diseases. Chapters guide the reader through the application of hypoxic conditions, to the techniques of molecular biology, biochemical, cell biology, genomic,bioinformatic, metabolic, and animal studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Hypoxia: Methods and Protocols aims to provide a valuable set of tools that can be used to study hypoxia and beyond. Table of Contents Whole Genome Sequencing-based Discovery of Structural Variants in Glioblastoma.- Whole Genome DNA Methylation Analysis of Human Glioblastoma using Illumina Bead Arrays.- Establishing Primary Human Glioblastoma Adherent Cultures from Operative Specimens.- Establishing Primary Human Glioblastoma Tumorsphere Cultures from Operative Specimens.- Isolation of GBM Stem Cells with Flow Cytometry.- Lentiviral Transduction of Primary Human Glioblastoma Cultures.- Selective Targeting of CD133-expressing Glioblastoma Stem Cells using Lentiviral Vectors.- Intracellular pH Measurements in Glioblastoma Cells using the pH-sensitive Dye BCECF.- Induction and Assessment of Hypoxia in Glioblastoma Cells in vitro.- Metabolomic Analysis of Glioma Cells Using Nanoflow Liquid Chromatography-tandem Mass Spectrometry.- Sample Preparation for Relative Quantitation of Proteins Using Tandem Mass Tags (TMT) and Mass Spectrometry (MS).- Single-Cell RNA Sequencing of Glioblastoma Cells.- Evaluation of Radioresponse and Radiosensitizers in Glioblastoma Organotypic Cultures.- Orthotopic Patient-derived Glioblastoma Xenografts in Mice.- Bioluminescent In Vivo Imaging of Orthotopic Glioblastoma Xenografts in Mice.- Evaluation of Vascularity, Blood Perfusion, and Oxygen Tension in Tumor Xenografts with Fluorescent Microscopy.- Probing Glioblastoma Tissue Heterogeneity with Laser Capture Microdissection.- Flow Cytometric Identification of Tumor-infiltrating Lymphocytes from Glioblastoma.- Modeling Glioma with Human Embryonic Stem Cell-derived Neural Lineages. Feature Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Details ISBN 1493976648 Publisher Humana Press Inc. Series Methods in Molecular Biology Year 2018 Edition 1st ISBN-10 1493976648 ISBN-13 9781493976645 Format Hardcover Imprint Humana Press Inc. Subtitle Methods and Protocols Place of Publication Totowa, NJ Country of Publication United States DEWEY 571.614 Edited by L. Eric Huang Pages 302 Illustrations 63 Illustrations, color; 10 Illustrations, black and white; XII, 302 p. 73 illus., 63 illus. in color. Publication Date 2018-01-13 Short Title Hypoxia Language English DOI 10.1007/978-1-4939-7665-2 Series Number 1742 AU Release Date 2018-01-13 NZ Release Date 2018-01-13 US Release Date 2018-01-13 UK Release Date 2018-01-13 Author L. Eric Huang Birth 1972 Affiliation Solicitor (Non-Practising) Senior Lecturer (Part Time) Staffordshire University Position Professor of Comparative Literature Qualifications JD, PhD, MSSA Edition Description 1st ed. 2018 Alternative 9781493985388 Audience Tertiary & Higher Education Country of Origin NL Product Class Description Life Sciences: General We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! 30 DAY RETURN POLICY No questions asked, 30 day returns! FREE DELIVERY No matter where you are in the UK, delivery is free. SECURE PAYMENT Peace of mind by paying through PayPal and eBay Buyer Protection TheNile_Item_ID:137721054;

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