Hydrocarbon and Lipid Microbiology Protocols: Genetic, Genomic and System Analys

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The Nile on eBay FREE SHIPPING UK WIDE Hydrocarbon and Lipid Microbiology Protocols by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales This Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities. FORMAT Paperback LANGUAGE English CONDITION Brand New Publisher Description This Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities. Methods are described for the creation and bioinformatic assessment of metagenomic and metatranscriptomic libraries, and for metaproteomic analyses, which provide important insights into the metabolic potential and interactions of community members. These in turn lead to specific hypotheses concerning the functional contributions of individual populations in the community, which may be investigated by the stable isotope probing approaches described in this Volume, making it possible to identify those community members primarily responsible for particular functions. Methods for the direct extraction of proteins from environmental samples for sequencing and activity tests are presented, providing a broad overview of prevailing metabolic activities and of the types of microbe involved in them. Protocols for the analysis of nutrient flow through microbial communities and for the modelling of dynamic physiological interactions in communities are also provided. Lastly, the book presents a protocol for the quantitative assessment of permissiveness for the transfer of conjugative plasmids, important agents of physiological change and evolution in microbial communities. Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes. Back Cover This Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities. Methods are described for the creation and bioinformatic assessment of metagenomic and metatranscriptomic libraries, and for metaproteomic analyses, which provide important insights into the metabolic potential and interactions of community members. These in turn lead to specific hypotheses concerning the functional contributions of individual populations in the community, which may be investigated by the stable isotope probing approaches described in this Volume, making it possible to identify those community members primarily responsible for particular functions. Methods for the direct extraction of proteins from environmental samples for sequencing and activity tests are presented, providing a broad overview of prevailing metabolic activities and of the types of microbe involved in them. Protocols for the analysis of nutrient flow through microbial communities and for the modelling of dynamic physiological interactions in communities are also provided. Lastly, the book presents a protocol for the quantitative assessment of permissiveness for the transfer of conjugative plasmids, important agents of physiological change and evolution in microbial communities. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes. Table of Contents Introduction to genetic, genomic and system analyses for communities.- Genomic analysis of pure cultures and communities.- Protocols for metagenomic library generation and analysis in petroleum hydrocarbon microbe systems.- Preparation and analysis of metatranscriptomic libraries in petroleum hydrocarbon microbe systems.- Prokaryotic metatranscriptomics.- Defining a pipeline for metaproteomic analyses.- Generating enriched metagenomes from active microorganisms with DNA stable isotope probing.- DNA and RNA-based stable isotope probing of hydrocarbon degraders.- Protein stable isotope probing (Protein-SIP).- Protein extraction from contaminated soils and sediments.- Analysis of the regulation of the rate of hydrocarbon and nutrient flow through microbial communities.- Physiological modelling of complex bacterial communities.- Protocol for evaluating the permissiveness of bacterial communities towards conjugal plasmids by quantification and isolation of transconjugants. Feature Offers readily-reproducible step-by-step laboratory methods Provides helpful tips and tricks supporting the protocols Includes troubleshooting advice Contains practical laboratory guidance to promote successful results Details ISBN 3662570629 ISBN-10 3662570629 ISBN-13 9783662570623 Format Paperback Series Springer Protocols Handbooks Subtitle Genetic, Genomic and System Analyses of Communities Edited by Kenneth N. Timmis DEWEY 660.62 Pages 288 Year 2018 Imprint Springer-Verlag Berlin and Heidelberg GmbH & Co. K Place of Publication Berlin Country of Publication Germany Illustrations 30 Illustrations, color; 20 Illustrations, black and white; X, 288 p. 50 illus., 30 illus. in color. Publication Date 2018-04-30 Short Title Hydrocarbon and Lipid Microbiology Protocols Language English UK Release Date 2018-04-30 Author Balbina Nogales Publisher Springer-Verlag Berlin and Heidelberg GmbH & Co. KG Alternative 9783662504499 Audience Professional & Vocational Country of Origin NL Product Class Description Life Sciences: General Edition Description Softcover Reprint of the Original 1st 2017 ed. We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! 30 DAY RETURN POLICY No questions asked, 30 day returns! FREE DELIVERY No matter where you are in the UK, delivery is free. SECURE PAYMENT Peace of mind by paying through PayPal and eBay Buyer Protection TheNile_Item_ID:137617707;