Animal Cell Culture Techniques by Martin Clynes (English) Paperback Book

Description

The Nile on eBay FREE SHIPPING UK WIDE Animal Cell Culture Techniques by Martin Clynes This manual on cell culture techniques addresses a variety of molecular and cellular biological questions, offering physiological conditions whilst avoiding using laboratory animals. In addition to basic techniques, a range of specialised practical protocols are included. FORMAT Paperback LANGUAGE English CONDITION Brand New Publisher Description Cell culture techniques allow a variety of molecular and cellular biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a range of specialised practical protocols covering the following areas are included: cell proliferation and death; in-vitro models models for cell differentiation; in-vitro models for toxicology and pharmacology; industrial application of animal cell culture; and genetic manipulation and analysis of human and animal cells in culture. Back Cover Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture. Table of Contents I General Methods.- 1 Cloning Animal Cells.- 2 Cell Culture and Diagnostic Virology.- 3 Screening for Mycoplasma Contamination in Animal Cell Cultures.- 4 Serum-Free Media.- 5 Screening Culture Media and Sera for Endotoxin Testing.- 6 Generation of Monoclonal Antibodies and Characterization of Novel Antibodies by Western Blotting and Immunocytochemistry.- 7 The Generation of Long-Term Human Antibody-Producing Cell Lines Using the Epstein-Barr Virus.- 8 Primary Culture.- II Cell proliferation and Death.- 9 Baculoviral Expression and Partial Purification of Cyclin/CDK Complexes.- 10 Methods for Studying Phosphoinositide Metabolism in Cultured Cells.- 11 Analysis of Cell Cycle and Cell Death Mechanisms.- III In Vitro Models for Cell Differentiation.- 12 Assessing Release of Secretory Products from Individual Cells.- 13 The Granulocyte/Macrophage Colony-Forming Unit Assay.- 14 Investigations into Cell Differentiation Using Cells in Culture.- 15 Cell Culture Models for Mononuclear Phagocytes.- 16 The Human Leukemia Cell Line HL-60 as a Cell Culture Model To Study Neutrophil Functions and Inflammatory Cell Responses.- 17 Cell Culture Models for Polarized Epithelial Monolayers.- 18 Ion Channels and Cell Signaling in Cell Cultures.- 19 Isolation, Cultivation and Differentiation of Lung Type II Epithelial Cells.- 20 Culture Systems for Hepatocytes for Use in Toxicology and Differentiation Studies.- IV In Vitro Models for Toxicology and Pharmacology.- 21 Cellular Models for In Vitro Toxicity Testing.- 22 Miniaturized In Vitro Methods in Toxicity Testing.- 23 Investigation of Anti-tumor Drug Accumulation and Efflux in Cell Culture.- 24 Current Status of Genetically Engineered In Vitro Systems for Biotransformation.- V Industrial Application of Animal Cell Culture.- 25 Scale-Up of Animal Cell Cultures.- 26 Purification of Monoclonal Antibodies Produced by Animal Cells in Culture.- 27 Biosafety Assessment of Cultured Animal Cells Used for Biopharmaceutical Production.- 28 Cytokine Cell Culture Assays.- VI Genetic Manipulation and Analysis of Human and Animal Cells in Culture.- 29 Antisense Methods in Cell Culture.- 30 Transfection with Ribozymes to Investigate the Role of Specific Gene Expression.- 31 Methods for Studying the Transcription and DNase I Hypersensitive Sites of Genes in Cells in Culture.- 32 Analysis of Specific and Differentially Expressed mRNAs in Cell Culture.- 33 Human Gene Therapy: Review and Outlook. Promotional Springer Book Archives Long Description Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture. Feature Clearly structured protocols for direct use in the laboratory Step-by step instructions with full practical details and background information Several of the reliable well-tested protocols are contributed by authors from the renowned Irish National Cell and Tissue Culture Centre Description for Sales People This laboratory manual provides a variety of practical protocols for anyone using animal cell culture techniques in a laboratory. Molecular and cell biological questions can be addressed whilst avoiding the use of laboratory animals. Details ISBN 3540630082 Publisher Springer-Verlag Berlin and Heidelberg GmbH & Co. KG Series Springer Lab Manuals Year 1998 ISBN-10 3540630082 ISBN-13 9783540630081 Format Paperback Imprint Springer-Verlag Berlin and Heidelberg GmbH & Co. K Place of Publication Berlin Country of Publication Germany DEWEY 571.6381 Short Title ANIMAL CELL CULTURE TECHNIQUES Language English Media Book Edited by Martin Clynes Pages 618 DOI 10.1604/9783540630081;10.1007/978-3-642-80412-0 Publication Date 1998-06-16 Author Martin Clynes Edition Description Softcover reprint of the original 1st ed. 1998 Audience Postgraduate, Research & Scholarly Country of Origin DE Product Class Description Life Sciences: General Alternative 9783642804120 Illustrations 34 Illustrations, black and white We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! 30 DAY RETURN POLICY No questions asked, 30 day returns! FREE DELIVERY No matter where you are in the UK, delivery is free. SECURE PAYMENT Peace of mind by paying through PayPal and eBay Buyer Protection TheNile_Item_ID:137662238;